Acetaldehyde dehydrogenase 2 (ALDH2) is a key enzyme in the catabolism of alcohol in vivo, and it also has the activity of nitroesterase, which is the key enzyme for the formation of nitric oxide, an effective metabolite of nitroglycerin. By detecting the genotype of ALDH2 in patients,the kit provides auxiliary diagnosis for patients with abnormal alcohol metabolism and effective clinical reference for the use of nitroglycerin.